• Catalog Number:

  E1A8449A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human VCP around the phosphorylation site of serine 352 (P-N-SP-I-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P55072

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  15S Mg(2+)-ATPase p97 subunit; TER ATPase; TERA; Transitional endoplasmic reticulum ATPase; Valosin containing protein; VCP

• Specificity Sensitivity:

  VCP (Phospho-Ser352) antibody detects endogenous levels of VCP only when phosphorylated at serine 352.

• References:

  Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000). Lamerdin J.E., Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases. Gevaert K., Nat. Biotechnol. 21:566-569(2003).

• All Sites:

  Human: Ser352; Mouse: Ser352; Rat: Ser352

Western blot analysis of extracts from 3T3 cells, treated with starved (24hours), using VCP (Phospho-Ser352) antibody.