• Catalog Number:

  E1A8444A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human UBF around the phosphorylation site of serine 484 (P-E-SP-P-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P17480

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  Autoantigen NOR-90; Nucleolar transcription factor 1; TCFUBF; UBF-1; UBF1; UBTF; Upstream binding factor 1

• Specificity Sensitivity:

  UBF (Phospho-Ser484) antibody detects endogenous levels of UBF only when phosphorylated at serine 484.

• References:

  Jantzen H.M., Nature 344:830-836(1990). Chan E.K.L., J. Exp. Med. 174:1239-1244(1991). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser484; Mouse: Ser484; Rat: Ser484

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using UBF (Phospho-Ser484) antibody. Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using UBF (Phospho-Ser484) antibody.