• Catalog Number:

  E1A8438A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human TOB1 around the phosphorylation site of serine 164 (A-V-SP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatographyusing non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P50616

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  TOB; Transducer of erbB-2 1

• Specificity Sensitivity:

  TOB1 (Phospho-Ser164) antibody detects endogenous levels of TOB1 only when phosphorylated at serine 164.

• References:

  Matsuda S., Oncogene 12:705-713(1996). Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Ser164; Mouse: Ser164; Rat: Ser164

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody. Western blot analysis of extracts from HT-29 cells, treated with serum (20%, 15mins), using TOB1 (Phospho-Ser164) antibody.