• Catalog Number:

  E1A8425A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SREBP-1 around the phosphorylation site of serine 439 (Q-S-SP-P-L).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%);Rat (Identities = 90%, Positives = 100%)

• SwissProtNo:

  P36956

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  SRBP1; SREBF1; SREBP1; sterol regulatory element-binding protein 1; sterol regulatory element-binding transcription factor 1

• Specificity Sensitivity:

  SREBP-1 (Phospho-Ser439) antibody detects endogenous levels of SREBP-1 only when phosphorylated at serine 439.

• References:

  Yokoyama C., Cell 75:187-197(1993). Hua X., Genomics 25:667-673(1995). Olsen J.V., Cell 127:635-648(2006).

• All Sites:

  Human: Ser439; Rat: Ser432

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using SREBP-1 (Phospho-Ser439) antibody. Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using SREBP-1 (Phospho-Ser439) antibody.