• Catalog Number:

  E1A8423A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SPHK2 around the phosphorylation site of threonine 614 (P-L-TP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q9NRA0

• Applications:

  IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 2.7.1.-; EC 2.7.1.91; SK 2; Sphingosine kinase 2; SPK 2

• Specificity Sensitivity:

  SPHK2 (Phospho-Thr614) antibody detects endogenous levels of SPHK2 only when phosphorylated at threonine 614.

• References:

  Liu H., J. Biol. Chem. 275:19513-19520(2000). Wiemann S., Genome Res. 11:422-435(2001). Ota T., Nat. Genet. 36:40-45(2004).

• All Sites:

  Human: Thr614; Mouse: Thr578; Rat: Thr577

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using SPHK2 (Phospho-Thr614) antibody. Nibrin (Phospho-Ser278) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.