• Catalog Number:

  E1A8413A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human S6 Ribosomal Protein around the phosphorylation site of serine 235and 236 (R-L-SP-SP-L-R).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P62753

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  40S ribosomal protein S6; Phosphoprotein NP33; ribosomal protein S6; RPS6; RS6

• Specificity Sensitivity:

  S6 Ribosomal Protein (Phospho-Ser235+Ser236) antibody detects endogenous levels of S6 Ribosomal Protein only when phosphorylated at serine 235&236.

• References:

  Lott J.B., Gene 65:31-39(1988). Heinze H., J. Biol. Chem. 263:4139-4144(1988). Antoine M., Hum. Mol. Genet. 1:565-570(1992).

• All Sites:

  Human: Ser235+Ser236; Mouse: Ser235+Ser236; Rat: Ser235+Ser236

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using S6 Ribosomal Protein (Phospho-Ser235+Ser236) antibody. Western blot analysis of extracts from 293 cells treated with TNF, using S6 Ribosomal Protein (Phospho-Ser235+Ser236) antibody.