• Catalog Number:

  E1A8412A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human RFWD2 around the phosphorylation site of serine 385 (T-A-SP-Q-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q8NHY2

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  Constitutive photomorphogenesis protein 1 homolog; COP1; EC 6.3.2.-; hCOP1; RING finger and WD repeat domain protein 2; Ubiquitin- protein ligase COP1

• Specificity Sensitivity:

  RFWD2 (Phospho-Ser387) antibody detects endogenous levels of RFWD2 only when phosphorylated at serine 385.

• References:

  Yi C., BMC Cell Biol. 3:30-30(2002). Wertz I.E., Science 303:1371-1374(2004). Ota T., Nat. Genet. 36:40-45(2004).

• All Sites:

  Human: Ser387; Mouse: Ser389

Western blot analysis of extracts from K562 cells, treated with UV (15mins), using RFWD2 (Phospho-Ser387) antibody.