• Catalog Number:

  E1A8391A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p47 phox around the phosphorylation site of serine 345 (P-Q-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P14598

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-1; NCF-47K; NCF1; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; Neutrophil NADPH oxidase factor 1; P47 phox; p47-phox

• Specificity Sensitivity:

  p47 phox (Phospho-Ser345) antibody detects endogenous levels of p47 phox only when phosphorylated at serine 345.

• References:

  Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989). Lomax K.J., Science 245:409-412(1989). Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).

• All Sites:

  Human: Ser345

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody. Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using p47 phox (Phospho-Ser345) antibody.