• Catalog Number:

  E1A8386A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NFAT5/TonEBP around the phosphorylation site of serine 155 (D-N-SP-R-M).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O94916

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  KIAA0827; NF-AT5; nuclear factor of activated T cells 5; T cell transcription factor NFAT5; TonE-binding protein; TonEBP; tonicity-responsive enhancer-binding protein

• Specificity Sensitivity:

  NFAT5/TonEBP (Phospho-Ser155) antibody detects endogenous levels of NFAT5/TonEBP only when phosphorylated at serine 155.

• References:

  Nagase T., DNA Res. 5:355-364(1998). Lopez-Rodriguez C., Proc. Natl. Acad. Sci. U.S.A. 96:7214-7219(1999). Miyakawa H., Proc. Natl. Acad. Sci. U.S.A. 96:2538-2542(1999).

• All Sites:

  Human: Ser155; Mouse: Ser155

Western blot analysis of extracts from HeLa cells, treated with forskolin (40nM, 30mins), using NFAT5/TonEBP (Phospho-Ser155) antibody.