• Catalog Number:

  E1A8383A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Neuro D around the phosphorylation site of serine 274 (P-L-SP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q13562

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  beta2; NDF1; NEUROD; NeuroD1; Neurogenic differentiation factor 1

• Specificity Sensitivity:

  Neuro D (Phospho-Ser274) antibody detects endogenous levels of Neuro D only when phosphorylated at serine 274.

• References:

  Tamimi R., Genomics 34:418-421(1996). Yokoyama M., Brain Res. Mol. Brain Res. 42:135-139(1996). Miyachi T., Brain Res. Mol. Brain Res. 69:223-231(1999).

• All Sites:

  Human: Ser274; Mouse: Ser274; Rat: Ser274

Western blot analysis of extracts from HeLa cells, treated with UV (15mins), using Neuro D (Phospho-Ser274) antibody.