• Catalog Number:

  E1A8372A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MKP-1/2 around the phosphorylation site of serine 296/318 (I-I-SP-P-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P28562/Q13115

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  3CH134; CL100; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1; DUSP1; EC 3.1.3.16; EC 3.1.3.48; MAP kinase phosphatase-1; MKP1; Protein-tyrosine phosphatase 3CH134; Protein-tyrosine phosphatase CL100; Protein-tyrosine phosphatase ERP; PTPN10; PTPN16; VH1

• Specificity Sensitivity:

  MKP-1/2 (Phospho-Ser296/318) antibody detects endogenous levels of MKP-1/2 only when phosphorylated at serine 296/318.

• References:

  Keyes S.M., Nature 359:644-647(1992). Kwak S.P., J. Biol. Chem. 269:3596-3604(1994). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser296/318; Mouse: Ser296/322; Rat: Ser296/319

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MKP-1/2 (Phospho-Ser296/318) antibody. Western blot analysis of extracts from K562 cells, treated with heat shock, using MKP-1/2 (Phospho-Ser296/318) antibody.