• Catalog Number:

  E1A8369A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MDM4 around the phosphorylation site of serine 367 (T-I-SP-A-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O15151

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  double minute 4 protein; Mdm2-like p53-binding protein; MDMX; p53-binding protein Mdm4

• Specificity Sensitivity:

  MDM4 (Phospho-Ser367) antibody detects endogenous levels of MDM4 only when phosphorylated at serine 367.

• References:

  Shvarts A., Genomics 43:34-42(1997). Gregory S.G., Nature 441:315-321(2006). Sharp D.A., J. Biol. Chem. 274:38189-38196(1999).

• All Sites:

  Human: Ser367; Mouse: Ser367; Rat: Ser368

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using MDM4 (Phospho-Ser367) antibody. Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MDM4 (Phospho-Ser367) antibody.