• Catalog Number:

  E1A8363A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of serine 272 (A-I-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P49137

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  EC 2.7.11.1; kinase MAPKAPK2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPK2; MAPKAP kinase 2; MAPKAPK-2; MAPKAPK2; RPS6KC1

• Specificity Sensitivity:

  MAPKAPK2 (Phospho-Ser272) antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at serine 272.

• References:

  Zu Y.-L., Biochem. Biophys. Res. Commun. 200-1118-1124(1994). The MGC Project Team; Genome Res. 14:2121-2127(2004). Stokoe D., Biochem. J. 296:843-849(1993).

• All Sites:

  Human: Ser272; Mouse: Ser258; Rat: Ser258

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAPKAPK2 (Phospho-Ser272) antibody. Western blot analysis of extracts from RAW264.7 cells, treated with UV (15mins), using MAPKAPK2 (Phospho-Ser272) antibody.