• Catalog Number:

  E1A8350A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Gab2 around the phosphorylation site of serine 623 (PS-SP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 91%, Positives = 91%);Rat (Identities = 91%, Positives = 91%)

• SwissProtNo:

  Q9UQC2

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  GRB2-associated-binding protein 2; Growth factor receptor bound protein 2-associated protein 2; GRB2-associated binder 2; pp100; KIAA0571

• Specificity Sensitivity:

  Gab2 (Phospho-Ser623) antibody detects endogenous levels of Gab2 only when phosphorylated at serine 623.

• References:

  Nishida K., Blood 93:1809-1816(1999). Nagase T., DNA Res. 5:31-39(1998). Taylor T.D., Nature 440:497-500(2006).

• All Sites:

  Human: Ser623; Mouse: Ser612; Rat: Ser612

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Gab2 (Phospho-Ser623) antibody. Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using Gab2 (Phospho-Ser623) antibody.