• Catalog Number:

  E1A8349A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Gab2 around the phosphorylation site of tyrosine 643 (V-D-YP-V-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q9UQC2

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  GABJ; GRB2-associated binding protein 2; KIAA0571; pp100

• Specificity Sensitivity:

  Gab2 (Phospho-Tyr643) antibody detects endogenous levels of Gab2 only when phosphorylated at tyrosine 643.

• References:

  Nishida K., Blood 93:1809-1816(1999). Nagase T., DNA Res. 5:31-39(1998). Salomon A.R., Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003).

• All Sites:

  Human: Tyr643; Mouse: Tyr632; Rat: Tyr632

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Gab2 (Phospho-Tyr643) antibody. Western blot analysis of extracts from Jurkat cells, treated with IFN (2500U/ML, 30mins), using Gab2 (Phospho-Tyr643) antibody.