• Catalog Number:

  E1A8341A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EPB41 around the phosphorylation site of tyrosine 660/418 (N-I-YP-I-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P11171

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  4.1R; 41; band 4.1; E41P; EPB4.1; erythrocyte membrane protein band 4.1; P4.1; protein 4.1

• Specificity Sensitivity:

  EPB41 (Phospho-Tyr660/418) antibody detects endogenous levels of EPB41 only when phosphorylated at tyrosine 660/418.

• References:

  Conboy J.G., Proc. Natl. Acad. Sci. U.S.A. 83:9512-9516(1986). Tang T.K., Adv. Exp. Med. Biol. 241:81-95(1988). Tang T.K., Proc. Natl. Acad. Sci. U.S.A. 85:3713-3717(1988). ----------------------------------------------------------------------------

• All Sites:

  Human: Tyr660; Mouse: Tyr654

Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using EPB41 (Phospho-Tyr660/418) antibody.