• Catalog Number:

  E1A8340A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human eEF2K around the phosphorylation site of serine 359 (G-SP-P-R-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O00418

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  Calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; eEF-2 kinase; eEF-2K; EF2K; Elongation factor 2 kinase; kinase Eef2k

• Specificity Sensitivity:

  eEF2K (Phospho-Ser359) antibody detects endogenous levels of eEF2K only when phosphorylated at serine 359.

• References:

  Ryazanov A.G., Proc. Natl. Acad. Sci. U.S.A. 94:4884-4889(1997). Martin J., Nature 432:988-994(2004).

• All Sites:

  Human: Ser359; Mouse: Ser358; Rat: Ser358

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using eEF2K (Phospho-Ser359) antibody.