• Catalog Number:

  E1A8336A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cyclin D2 around the phosphorylation site of threonine 280 (A-S-TP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 90%, Positives = 100%);Rat (Identities = 90%, Positives = 100%)

• SwissProtNo:

  P30279

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  cyclin D2; G1/S-specific cyclin D2

• Specificity Sensitivity:

  Cyclin D2 (Phospho-Thr280) antibody detects endogenous levels of Cyclin D2 only when phosphorylated at threonine 280.

• References:

  Xiong Y., Genomics 13:575-584(1992). Palmero I., Oncogene 8:1049-1054(1993). Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Thr280; Mouse: Thr279; Rat: Thr279

Western blot analysis of extracts from COLO cells, treated with EGF (200ng/ml, 30mins), using Cyclin D2 (Phospho-Thr280) antibody.