• Catalog Number:

  E1A8334A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cyclin C around the phosphorylation site of serine 275 (N-G-SP-Q-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P24863

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  CG1C; cyclin C; SRB11

• Specificity Sensitivity:

  Cyclin C (Phospho-Ser275) antibody detects endogenous levels of Cyclin C only when phosphorylated at serine 275.

• References:

  Lew D.J., Cell 66:1197-1206(1991). Li H., Genomics 32:253-259(1996). Mungall A.J., Nature 425:805-811(2003).

• All Sites:

  Human: Ser275; Mouse: Ser275; Rat: Ser270

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Cyclin C (Phospho-Ser275) antibody. Western blot analysis of extracts from 3T3 cells, treated with UV (15mins), using Cyclin C (Phospho-Ser275) antibody.