Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Claudin 7 around the phosphorylation site of tyrosine 210 (S-K-E-YP-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Claudin 7 (Phospho-Tyr210) antibody detects endogenous levels of Claudin 7 only when phosphorylated at tyrosine 210.
References:
Keen T.J.; Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
All Sites:
Human: Tyr210; Mouse: Tyr210; Rat: Tyr210
Western blot analysis of extracts from rat liver cells, using Claudin 7 (Phospho-Tyr210) antibody.