• Catalog Number:

  E1A8294A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ATF-2 around the phosphorylation site of serine 472 (A-L-SP-Q-I).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P15336

• Applications:

  IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  Activating 2; ATF2; cAMP response element binding protein CRE- BP1; CREB2; CREBP1; Cyclic-AMP-dependent ATF-2; HB16; MXBP protein

• Specificity Sensitivity:

  ATF-2 (Phospho-Ser472) antibody detects endogenous levels of ATF-2 only when phosphorylated at serine 472.

• References:

  Maekawa T., EMBO J. 8:2023-2028(1989). Yang L., J. Immunol. 158:2522-2525(1997). Kara C.J., Mol. Cell. Biol. 10:1347-1357(1990).

• All Sites:

  Human: Ser472; Mouse: Ser472; Rat: Ser472

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using ATF-2 (Phospho-Ser472) antibody.