• Catalog Number:

  E1A8287A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AML1 around the phosphorylation site of serine 435 (S-N-SP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q01196

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  Acute myeloid leukemia 1 protein; CBF-alpha 2; CBFA2; Core-binding factor, alpha 2 subunit; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer binding protein 2 alpha B subunit; RUN1; Runt-related transcription factor 1; RUNX1; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit

• Specificity Sensitivity:

  AML1 (Phospho-Ser435) antibody detects endogenous levels of AML1 only when phosphorylated at serine 435.

• References:

  Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991). Sacchi N., Genes Chromosomes Cancer 11:226-236(1994). Nucifora G., Blood 81:2728-2734(1993).

• All Sites:

  Human: Ser435; Mouse: Ser435; Rat: Ser435

Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using AML1 (Phospho-Ser435) antibody.