• Catalog Number:

  E1A8278A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GRK2 around the phosphorylation site of serine 685 (R-G-SP-A-N).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 91%, Positives = 100%);Rat (Identities = 91%, Positives = 100%)

• SwissProtNo:

  P25098

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  ADRBK1; ARBK1; BARK; BARK1; beta-adrenergic receptor kinase 1; beta-ARK-1; EC 2.7.11.15; G protein receptor kinase 2; G- protein coupled receptor kinase 2; kinase GRK2

• Specificity Sensitivity:

  GRK2 (Phospho-Ser685) antibody detects endogenous levels of GRK2 only when phosphorylated at serine 685.

• References:

  Benovic J.L., FEBS Lett. 283:122-126(1991). Chuang T.T., J. Biol. Chem. 267:6886-6892(1992). Penn R.B., J. Biol. Chem. 269:14924-14930(1994).

• All Sites:

  Human: Ser685; Mouse: Ser685; Rat: Ser685

Western blot analysis of extracts from HT-29 cells, treated with insulin (0.01U/ml, 15mins), using GRK2 (Phospho-Ser685) antibody.