• Catalog Number:

  E1A8276A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human WWOX around the phosphorylation site of tyrosine 33 (W-V-YP-Y-A).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q96KM3

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  Aberrant WW domain-containing oxidoreductase; EC 1.1.1.-; FOR; WOX1; WW domain-containing oxidoreductase

• Specificity Sensitivity:

  WWOX (Phospho-Tyr33) antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.

• References:

  Bednarek A.K., Cancer Res. 60:2140-2145(2000). Ried K., Hum. Mol. Genet. 9:1651-1663(2000). Bednarek A.K., Cancer Res. 61:8068-8073(2001).

• All Sites:

  Human: Tyr33; Mouse: Tyr33

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody. Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using WWOX (Phospho-Tyr33) antibody.