• Catalog Number:

  E1A8270A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ATR around the phosphorylation site of serine 428 (G-I-SP-P-K).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q13535

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  ataxia telangiectasia and Rad3-related protein; EC 2.7.11.1; FRAP-related protein; FRP1; kinase ATR; protein kinase ATR

• Specificity Sensitivity:

  ATR (Phospho-Ser428) antibody detects endogenous levels of ATR only when phosphorylated at serine 428.

• References:

  Bentley N.J., EMBO J. 15:6641-6651(1996). Cimprich K.A., Proc. Natl. Acad. Sci. U.S.A. 93:2850-2855(1996). Mannino J.L., Gene 272:35-43(2001).

• All Sites:

  Human: Ser428

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ATR (Phospho-Ser428) antibody. ATR (Phospho-Ser428) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.