• Catalog Number:

  E1A8262A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HP1α around the phosphorylation site of serine 92 (R-K-SP-N-F).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P45973

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  antigen p25; chromobox protein homolog 5; heterochromatin protein 1 alpha; HP1 alpha; HP1A

• Specificity Sensitivity:

  HP1α (Phospho-Ser92) antibody detects endogenous levels of HP1α only when phosphorylated at serine 92.

• References:

  Saunders W.S., J. Cell Sci. 104:573-582(1993). Ye Q., J. Biol. Chem. 271:14653-14656(1996). Ye Q., J. Biol. Chem. 272:14983-14989(1997).

• All Sites:

  Human: Ser92

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HP1α (Phospho-Ser92) antibody. HP1α (Phospho-Ser92) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.