• Catalog Number:

  E1A8260A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PYK2 around the phosphorylation site of tyrosine 579 (E-D-YP-Y-K).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q14289

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  CADTK; CAK beta; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; EC 2.7.10.2; FADK 2; FAK2; Focal adhesion kinase 2; kinase Pyk2; Proline-rich tyrosine kinase 2; Protein tyrosine kinase 2 beta; PTK2B; RAFTK; Related adhesion focal tyrosine kinase

• Specificity Sensitivity:

  PYK2 (Phospho-Tyr579) antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 579.

• References:

  Lev S., Nature 376:737-745(1995). Herzog H., Genomics 32:484-486(1996). Sasaki H., J. Biol. Chem. 270:21206-21219(1995).

• All Sites:

  Human: Tyr579; Mouse: Tyr579; Rat: Tyr579

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody. Western blot analysis of extracts from 3T3 cells, using PYK2 (Phospho-Tyr579) antibody.