• Catalog Number:

  E1A8257A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NMDAR2B around the phosphorylation site of tyrosine 1336 (S-P-YP-A-H).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q13224

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  Glutamate [NMDA] receptor subunit epsilon 2 precursor; GRIN2B; hNR3; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDE2; NME2; NR2B; NR3

• Specificity Sensitivity:

  NMDAR2B (Phospho-Tyr1336) antibody detects endogenous levels of NMDAR2B only when phosphorylated at tyrosine 1336.

• References:

  Adams S.L., Biochim. Biophys. Acta 1260:105-108(1995). Hess S.D., J. Pharmacol. Exp. Ther. 278:808-816(1996). Mandich P., Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Tyr1336; Mouse: Tyr1336; Rat: Tyr1336

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using NMDAR2B (Phospho-Tyr1336) antibody. Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using NMDAR2B (Phospho-Tyr1336) antibody.