• Catalog Number:

  E1A8254A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ITCH around the phosphorylation site of tyrosine 420 (F-I-YP-G-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q96J02

• Applications:

  IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  AIF4; AIP4; atrophin-1-interacting protein 4; EC 6.3.2.-; Itchy homolog E3 ubiquitin protein ligase; NAPP1; NFE2-associated polypeptide 1; ubiquitin protein ligase ITCH

• Specificity Sensitivity:

  ITCH (Phospho-Tyr420) antibody detects endogenous levels of ITCH only when phosphorylated at tyrosine 420.

• References:

  Chen X., Genomics 73:238-241(2001). Deloukas P., Nature 414:865-871(2001). Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998).

• All Sites:

  Human: Tyr420; Mouse: Tyr381

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody. ITCH (Phospho-Tyr420) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.