• Catalog Number:

  E1A8241A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GAB2 around the phosphorylation site of serine 159 (K-S-SP-A-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q9UQC2

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  GABJ; GAB2-associated binding protein 2; KIAA0571; pp100

• Specificity Sensitivity:

  GAB2 (Phospho-Ser159) antibody detects endogenous levels of GAB2 only when phosphorylated at serine 159.

• References:

  Nishida K., Blood 93:1809-1816(1999). Nagase T., DNA Res. 5:31-39(1998). Taylor T.D., Nature 440:497-500(2006). ----------------------------------------------------------------------------

• All Sites:

  Human: Ser159; Mouse: Ser160; Rat: Ser160

Western blot analysis of extracts from HT-29 cells, treated with serum (20%, 15mins), using GAB2 (Phospho-Ser159) antibody.