Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human DDR1 around the phosphorylation site of tyrosine 513 (P-A-YP-R-L).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
DDR1 (Phospho-Tyr513) antibody detects endogenous levels of DDR1 only when phosphorylated at tyrosine 513.
References:
di Marco E., J. Biol. Chem. 268:24290-24295(1993).
Johnson J.D., Proc. Natl. Acad. Sci. U.S.A. 90:5677-5681(1993).
Laval S., Cell Growth Differ. 5:1173-1183(1994).
All Sites:
Human: Tyr513; Mouse: Tyr511; Rat: Tyr510
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using DDR1 (Phospho-Tyr513) antibody.
Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3mM, 40mins), using DDR1 (Phospho-Tyr513) antibody.