• Catalog Number:

  E1A8237A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 216 (G-F-YP-I-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P12931

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  c-Src; C-SRC; EC 2.7.10.2; kinase Src; Neuronal SRC; p60-SRC; p60-Src; Src; SRC; SRC1

• Specificity Sensitivity:

  Src (Phospho-Tyr216) antibody detects endogenous levels of Src only when phosphorylated at tyrosine 216.

• References:

  Deloukas P., Nature 414:865-871(2001). Tanaka A., Mol. Cell. Biol. 7:1978-1983(1987). Anderson S.K., Mol. Cell. Biol. 5:1122-1129(1985).

• All Sites:

  Human: Tyr216; Mouse: Tyr221; Rat: Tyr216

Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 5mins), using Src (Phospho-Tyr216) antibody.