• Catalog Number:

  E1A8236A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HP1γ around the phosphorylation site of serine 93 (R-K-SP-L-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q13185

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  chromobox 3; chromobox 3 (HP1 gamma); HECH; heterochromatin protein 1 gamma; heterochromatin protein HP1 gamma; heterochromatin-like protein 1; HP1 gamma; HP1 gamma homolog; HP1-GAMMA; HP1Hs-gamma; modifier 2 protein

• Specificity Sensitivity:

  HP1γ (Phospho-Ser93) antibody detects endogenous levels of HP1γ only when phosphorylated at serine 93.

• References:

  Ye Q., J. Biol. Chem. 271:14653-14656(1996). Ye Q., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases. Koike N., FEBS Lett. 467:17-21(2000).

• All Sites:

  Human: Ser93; Mouse: Ser93; Rat: Ser93

Western blot analysis of extracts from K562 cells, treated with forskolin (40nM, 30mins), using HP1γ (Phospho-Ser93) antibody.