• Catalog Number:

  E1A8226A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FOS around the phosphorylation site of threonine 232 (V-A-TP-P-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 84%, Positives = 92%);Rat (Identities = 84%, Positives = 84%)

• SwissProtNo:

  P01100

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1: 20000

• Other Names:

  Cellular oncogene fos; FOS; G0/G1 switch regulatory protein 7; G0S7; Proto-oncogene protein c-fos

• Specificity Sensitivity:

  FOS (Phospho-Thr232) antibody detects endogenous levels of FOS only when phosphorylated at threonine 232.

• References:

  van Straaten F. Proc. Natl. Acad. Sci. U.S.A. 80:3183-3187(1983). Hai T., Genes Dev. 3:2083-2090(1989). Heilig R., Nature 421:601-607(2003).

• All Sites:

  Human: Thr232; Mouse: Thr232; Rat: Thr232

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FOS (Phospho-Thr232) antibody. Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 5mins), using FOS (Phospho-Thr232) antibody.