• Catalog Number:

  E1A8220A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Retinoic Acid Receptor α around the phosphorylation site of serine 77 (P-P-SP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P10276

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  NR1B1; RAR-alpha; Retinoic acid receptor alpha; retinoic acid receptor, alpha; RRA

• Specificity Sensitivity:

  Retinoic Acid Receptor α (Phospho-Ser77) antibody detects endogenous levels of Retinoic Acid Receptor α only when phosphorylated at serine 77.

• References:

  Giguere V., Nature 330:624-629(1987). Chen Z., Proc. Natl. Acad. Sci. U.S.A. 91:1178-1182(1994). Hjalt T.A.H., Mamm. Genome 10:528-529(1999).

• All Sites:

  Human: Ser77; Mouse: Ser77; Rat: Ser74

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Retinoic Acid Receptor α (Phospho-Ser77) antibody. Western blot analysis of extracts from Jurkat cells treated with PMA (125ng/ml, 30mins), using Retinoic Acid Receptor α (Phospho-Ser77) antibody.