Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human BLNK around the phosphorylation site of tyrosine 84 (E-M-YP-V-M).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
BLNK (Phospho-Tyr84) antibody detects endogenous levels of BLNK only when phosphorylated at tyrosine 84.
References:
Fu C., Immunity 9:93-103(1998).
Minegishi Y., Science 286:1954-1957(1999).
The MGC Project Team; Genome Res. 14-2121-2127(2004).
All Sites:
Human: Tyr84; Mouse: Tyr84; Rat: Tyr84
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using BLNK (Phospho-Tyr84) antibody.
Western blot analysis of extracts from K562 cells, treated with starved (24hours), using BLNK (Phospho-Tyr84) antibody.