• Catalog Number:

  E1A8205A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Vitamin D3 Receptor around the phosphorylation site of serine 51 (R-R-SP-M-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P11473

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  1,25-dihydroxyvitamin D3 receptor; NR1I1; vitamin D receptor; vitamin D3 receptor

• Specificity Sensitivity:

  Vitamin D3 Receptor (Phospho-Ser51) antibody detects endogenous levels of Vitamin D3 Receptor only when phosphorylated at serine 51.

• References:

  Baker A.R., Proc. Natl. Acad. Sci. U.S.A. 85:3294-3298(1988). Goto H., Biochim. Biophys. Acta 1132:103-108(1992). Miyamoto K., Mol. Endocrinol. 11:1165-1179(1997).

• All Sites:

  Human: Ser51; Mouse: Ser51; Rat: Ser51

Western blot analysis of extracts from COLO cells, treated with Insulin (0.01U/ml, 15mins), using Vitamin D3 Receptor (Phospho-Ser51) antibody.