• Catalog Number:

  E1A8204A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Androgen Receptor around the phosphorylation site of tyrosine 363 (D-Y-YP-N-F).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%)

• SwissProtNo:

  P10275

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  ANDR; Androgen receptor; DHTR; Dihydrotestosterone receptor; NR3C4

• Specificity Sensitivity:

  Androgen Receptor (Phospho-Tyr363) antibody detects endogenous levels of Androgen Receptor only when phosphorylated at tyrosine 363.

• References:

  Lubahn D.B., Mol. Endocrinol. 2:1265-1275(1988). Chang C., Proc. Natl. Acad. Sci. U.S.A. 85:7211-7215(1988). Tilley W.D., Proc. Natl. Acad. Sci. U.S.A. 86:327-331(1989).

• All Sites:

  Human: Tyr363; Mouse: Tyr358; Rat: Tyr361

Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using Androgen Receptor (Phospho-Tyr363) antibody.