• Catalog Number:

  E1A8199A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human WEE1 around the phosphorylation site of serine 53 (E-D-SP-A-F).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P30291

• Applications:

  IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  EC 2.7.10.2; kinase WEE1; Wee1-like protein kinase; WEE1A; WEE1hu

• Specificity Sensitivity:

  WEE1 (Phospho-Ser53) antibody detects endogenous levels of WEE1 only when phosphorylated at serine 53.

• References:

  Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001). Lu R., J. Biol. Chem. 279:31164-31170(2004). Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).

• All Sites:

  Human: Ser53; Mouse: Ser52; Rat: Ser52

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using WEE1 (Phospho-Ser53) antibody. WEE1 (Phospho-Ser53) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.