• Catalog Number:

  E1A8182A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human RSK1/2/3/4 around the phosphorylation site of serine 221/227/218/232 (A-Y-SP-F-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q15418/P51812/Q15349/Q9UK32

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  90 kDa ribosomal protein S6 kinase 1; EC 2.7.11.1; kinase p90RSK1; KS6A1; KS6AA; MAPKAP-K1a; p90-RSK 1; pp90RSK1; Ribosomal protein S6 kinase alpha 1; Ribosomal S6 kinase 1; RPS6KA1; RSK-1; RSK1; S6K-alpha 1

• Specificity Sensitivity:

  RSK1/2/3/4 (Phospho-Ser221/227/218/232) antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at serine 221/227/218/232.

• References:

  Moller D.E., Am. J. Physiol. 266:C351-C359(1994). Gregory S.G., Nature 441:315-321(2006). The MGC Project Team, Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser221/ Ser227/ Ser218/ Ser232; Mouse: Ser221/Ser 227/ Ser218/Ser 252

Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using RSK1/2/3/4 (Phospho-Ser221/227/218/232) antibody.