• Catalog Number:

  E1A8174A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKR around the phosphorylation site of threonine 258 (K-Y-TP-V-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P19525

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  ADRB2; E2AK2; EC 2.7.11.1; EIF2aK; EIF2AK2; Interferon-induced, double-stranded RNA-activated protein kinase; Interferon-inducible RNA-dependent protein kinase; kinase PKR; P1/eIF-2A protein kinase; p68 kinase; PRKR; TIK

• Specificity Sensitivity:

  PKR (Phospho-Thr258) antibody detects endogenous levels of PKR only when phosphorylated at threonine 258.

• References:

  Meurs E., Cell 62:379-390(1990). Thomis D.C., Virology 188:33-46(1992). Kuhen K.L., Gene 178:191-193(1996).

• All Sites:

  Human: Thr258

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKR (Phospho-Thr258) antibody. Western blot analysis of extracts from Jurkat cells, treated with starved (24hours), using PKR (Phospho-Thr258) antibody.