• Catalog Number:

  E1A8169A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKCB around the phosphorylation site of serine 661 (G-F-SP-Y-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P05771

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  EC 2.7.11.13; kinase PKC-beta; KPCB; PKC II; PKC-B; PKC-beta; PKC-II; PRKCB; PRKCB1; Protein kinase C, beta type

• Specificity Sensitivity:

  PKCB (Phospho-Ser661) antibody detects endogenous levels of PKCB only when phosphorylated at serine 661.

• References:

  Coussens L., Science 233:859-866(1986). Kubo K., FEBS Lett. 223:138-142(1987). Loftus B.J., Genomics 60:295-308(1999).

• All Sites:

  Human: Ser661; Mouse: Ser629; Rat: Ser661

Lane 1: The extracts from HeLa cells treated with heat shock using PKCB (Phospho-Ser661) antibody Lane 2: The extracts from HeLa cells treated with heat shock plus phosphopeptide using PKCB (Phospho-Ser661) antibody Lane 3: The extracts from HeLa cells treated with heat shock using PKCB (Ab-661) antibody