• Catalog Number:

  E1A8168A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PEK/PERK around the phosphorylation site of threonine 981 (R-H-TP-G-Q).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 92%);Rat (Identities = 92%, Positives = 92%)

• SwissProtNo:

  Q9NZJ5

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  E2AK3; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2-alpha kinase 3 precursor; HsPEK; kinase PERK; Pancreatic eIF2-alpha kinase; PEK; PERK; PRKR-like endoplasmic reticulum kinase

• Specificity Sensitivity:

  PEK/PERK (Phospho-Thr981) antibody detects endogenous levels of PEK/PERK only when phosphorylated at threonine 981.

• References:

  Shi Y., An J., J. Biol. Chem. 274:5723-5730(1999). Sood R., Biochem. J. 346:281-293(2000). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Thr981; Mouse: Thr980; Rat: Thr974

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using PEK/PERK (Phospho-Thr981) antibody. PEK/PERK (Phospho-Thr981) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.