• Catalog Number:

  E1A8151A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MYT1 around the phosphorylation site of serine 83 (R-V-SP-F-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q99640

• Applications:

  IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 2.7.11.1; kinase Myt1; Kinase MYT1; Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase; MYT1 kinase; Myt1 protein; PKMYT1; PMYT1

• Specificity Sensitivity:

  MYT1 (Phospho-Ser83) antibody detects endogenous levels of MYT1 only when phosphorylated at serine 83.

• References:

  Booher R.N., J. Biol. Chem. 272:22300-22306(1997). Liu F., Mol. Cell. Biol. 17:571-583(1997). Nousiainen M., Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).

• All Sites:

  Human: Ser83

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MYT1 (Phospho-Ser83) antibody. MYT1 (Phospho-Ser83) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.