• Catalog Number:

  E1A8149A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MSK2 around the phosphorylation site of threonine 568 (M-Q-TP-P-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 85%, Positives = 85%)

• SwissProtNo:

  O75676

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  EC 2.7.11.1; kinase MSK2; KS6A4; Ribosomal protein kinase B; RPS6KA4; RSK-B; Similar to ribosomal protein S6 kinase, 90kD, polypeptide 4

• Specificity Sensitivity:

  MSK2 (Phospho-Thr568) antibody detects endogenous levels of MSK2 only when phosphorylated at threonine 568.

• References:

  Pierrat B., J. Biol. Chem. 273-29661-29671(1998). Totoki Y., Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases. Deak M., EMBO J. 17:4426-4441(1998).

• All Sites:

  Human: Thr568; Mouse: Thr568

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using MSK2 (Phospho-Thr568) antibody. Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using MSK2 (Phospho-Thr568) antibody.