• Catalog Number:

  E1A8147A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MSK1 around the phosphorylation site of serine 212 (A-Y-SP-F-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O75582

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  90 kDa ribosomal protein S6 kinase, , polypeptide 5; EC 2.7.11.1; kinase MSK1; KS6A5; MSPK1; Nuclear mitogen- and stress-activated protein kinase-1; ribosomal protein S6 kinase, polypeptide V; RLPK; RPS6KA5

• Specificity Sensitivity:

  MSK1 (Phospho-Ser212) antibody detects endogenous levels of MSK1 only when phosphorylated at serine 212.

• References:

  Deak M., EMBO J. 17:4426-4441(1998). New L., J. Biol. Chem. 274:1026-1032(1999). Jiang C., Cytogenet. Cell Genet. 87:261-262(1999).

• All Sites:

  Human: Ser212; Mouse: Ser211

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MSK1 (Phospho-Ser212) antibody. Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 5mins), using MSK1 (Phospho-Ser212) antibody.