• Catalog Number:

  E1A8144A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MNK1 around the phosphorylation site of threonine 250 (I-T-TP-P-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9BUB5

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  kinase Mnk1; Map kinase interacting kinase; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine kinase 1; MKNK1

• Specificity Sensitivity:

  MNK1 (Phospho-Thr250) antibody detects endogenous levels of MNK1 only when phosphorylated at threonine 250.

• References:

  Fukunaga R., EMBO J. 16:1921-1933(1997). Knauf U., Mol. Cell. Biol. 21:5500-5511(2001). O'Loghlen A., Exp. Cell Res. 299:343-355(2004).

• All Sites:

  Human: Thr250; Mouse: Thr209; Rat: Thr197

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MNK1 (Phospho-Thr250) antibody.