• Catalog Number:

  E1A8141A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAP2K7 around the phosphorylation site of threonine 275 (A-K-TP-R-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O14733

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  c-Jun N-terminal kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 7; EC 2.7.12.2; JNK activating kinase 2; JNK kinase 2; JNKK 2; JNKK2; kinase MKK7; MAP kinase kinase 7; MAP2K7; MAPK/ERK kinase 7; MAPKK 7; MP2K7; PRKMK7

• Specificity Sensitivity:

  MAP2K7 (Phospho-Thr275) antibody detects endogenous levels of MAP2K7 only when phosphorylated at threonine 275.

• References:

  Wu Z.,Mol. Cell. Biol. 17:7407-7416(1997). Lu X.,J. Biol. Chem. 272:24751-24754(1997). The MGC Project Team;Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Thr275; Mouse: Thr291; Rat: Thr275

Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MAP2K7 (Phospho-Thr275) antibody.