• Catalog Number:

  E1A8140A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAP2K7 around the phosphorylation site of serine 271 (V-D-SP-K-A).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O14733

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  c-Jun N-terminal kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 7; EC 2.7.12.2; JNK activating kinase 2; JNK kinase 2; JNKK 2; JNKK2; kinase MKK7; MAP kinase kinase 7; MAP2K7; MAPK/ERK kinase 7; MAPKK 7; MP2K7; PRKMK7

• Specificity Sensitivity:

  MAP2K7 (Phospho-Ser271) antibody detects endogenous levels of MAP2K7 only when phosphorylated at serine 271.

• References:

  Wu Z., Mol. Cell. Biol. 17:7407-7416(1997). Lu X., J. Biol. Chem. 272:24751-24754(1997). Foltz I.N., J. Biol. Chem. 273:9344-9351(1998). -- ----------------------------------------------------------------------------------------------------------------------------------------------------

• All Sites:

  Human: Ser271; Mouse: Ser287; Rat: Ser271

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded humanbrain tissue using MAP2K7 (Phospho-Ser271) antibody. Lane 1: The extracts from 3T3 cells treated with insulin (0.01U/ml, 15mins) using MAP2K7 (Phospho-Ser271) antibody Lane 2: The extracts from 3T3 cells treated with insulin (0.01U/ml, 15mins) using MAP2K7 (Ab-271) antibody Lane 3: The extracts from 3T3 cells treated with insulin (0.01U/ml, 15mins) plus phosphopeptide using MAP2K7 (Phospho-Ser271) antibody