• Catalog Number:

  E1A8139A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAP2K3 around the phosphorylation site of threonine 222 (A-K-TP-M-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P46734

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  dual specificity mitogen-activated protein kinase kinase 3; EC 2.7.12.2; kinase MKK3; MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK3; MP2K3; PRKMK3

• Specificity Sensitivity:

  MAP2K3 (Phospho-Thr222) antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222.

• References:

  Derijard B., Science 267:682-685(1995). Moriguchi T., J. Biol. Chem. 271:26981-26988(1996). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Thr222; Mouse: Thr222; Rat: Thr222

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP2K3 (Phospho-Thr222) antibody. Western blot analysis of extracts from Jurkat cells, treated with serum (20%, 15mins), using MAP2K3 (Phospho-Thr222) antibody.